*Protein ID (NP or XP #) or Wolbachia#: TDR targetID: 3944
*Organism:Plasmodium falciparum
*Background/Disease Information: Plasmodium falciprum is commonly known to cause malaria in human. Malaria is aused by a parasite that is passed from one human to another by the bite of infected Anopheles mosquitoes. After infection, the parasites (called sporozoites) travel through the bloodstream to the liver, where they mature and release another form, the merozoites. The parasites enter the bloodstream and infect red blood cells.
Expression Information: upregulation ranking shows that this gene ranks in the mid 40-60% group of genes (In a form of early schizont and late trophozoite). Also, expression system is Escherichia coli bl21(de3).
Purification Method:
Image of protein (PyMol or etc):
P. falciparum dihydrofolate reductase-thymidylate synthase (DOI: 10.2210, UniProtKB: P13922).
*Protein ID (NP or XP #) or Wolbachia#: TDR targetID: 3944
*Organism: Plasmodium falciparum
*Background/Disease Information: Plasmodium falciprum is commonly known to cause malaria in human. Malaria is
aused by a parasite that is passed from one human to another by the bite of infected Anopheles mosquitoes. After infection, the parasites (called sporozoites) travel through the bloodstream to the liver, where they mature and release another form, the merozoites. The parasites enter the bloodstream and infect red blood cells.
Essentiality of this protein: It is very essential since it avoids the usage of antibiotics and other medications.
(http://www.ncbi.nlm.nih.gov/pubmed/16549005)
Complex of enzymes: not significantly complex
*EC#: 1.5.1.3
Link to BRENDA EC# page:
http://www.brenda-enzymes.org/php/result_flat.php4?ecno=1.5.1.3&Suchword=&organism%5B%5D=Plasmodium+falciparum&show_tm=0
Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):It is linked to sigma aldrich website. (http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Enzyme_Assay/dihydrofolatereductase.Par.0001.File.tmp/dihydrofolatereductase.pdf)
Structure Available (PDB or Homology model):
: http://www.pdb.org/pdb/explore/explore.do?structureId=1J3I
Target : see the link (http://tdrtargets.org/targets/view?gene_id=3944)
Current Inhibitors: listed below
Expression Information: upregulation ranking shows that this gene ranks in the mid 40-60% group of genes (In a form of early schizont and late trophozoite). Also, expression system is Escherichia coli bl21(de3).
Purification Method:
Image of protein (PyMol or etc):
http://www.pdb.org/pdb/explore/explore.do?structureId=1J3I
*length of your protein in Amino Acids =
Molecular Weight of your protein in kiloDaltons using the Expasy ProtParamwebsite =
Orthologs: Numerous but first 10 are:
Arabidopsis thaliana NP_195183 THY-2 (thymidylate synthase 2); dihydrofolate reductase/ th... | OrthoMCL
Arabidopsis thaliana NP_179230 THY-1 (THYMIDYLATE SYNTHASE 1); dihydrofolate reductase/ th... | OrthoMCL
Babesia bovis 3I3R B Chain B, X-Ray Structure Dihydrofolate ReductaseTHYMIDYLA... | OrthoMCL
Babesia bovis XP_001609606.1 dihydrofolate reductase/thymidilate synthase ... | OrthoMCL
Brugia malayi Bm1_33465 thymidylate synthase, putative... | OrthoMCL
Caenorhabditis elegans Y110A7A.4 CE24124 WBGene00022455 thymidylate synthase status:Confirmed... | OrthoMCL
Cryptosporidium hominis Chro.40506 chain A, crystal structure of Dhfr ... | OrthoMCL
Cryptosporidium muris CMU_010300 thymidylate synthase family protein ... | OrthoMCL
Cryptosporidium neoformas XP_567501 thymidylate synthase ... | OrthoMCL
Cryptosporidium parvum cgd4_4460 dihydrofolate reductase-thymidylate synthase ... | OrthoMCL
- Druggability: druggability index: 1 (0 to 1 range)
- By orthology to known druggable target
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- Additional Info.: Ki: 0.03 nM, IC50: 1820.0 nM
(TDR info: http://tdrtargets.org/targets/view?gene_id=3944)